 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isolation and Culture of Protoplasts from Different Explants of Cotton (Gossypium hirsutum L.)) ผู้เขียน: ดร.ประพนธ์ บุญรำพรรณ ,  Catherine Pannetier, Yves Chupeau สื่อสิ่งพิมพ์:pdf AbstractProtoplasts were isolated from callus, cotyledons, hypocotyls and leaves of cotton (Gossypium hirsutum L. var. Coker 310) using different digestion media. Protoplast yield per gram of fresh mass of leaves, callus cotyledons and hypocotyls was 22.2, 5.5, 1.7 and 1.1 million respectively. Protoplasts were most viable in basic medium in presence of thidiazuro 0.663 gm/l and 2, 4, 5-trichlorophenoxy acetic acid 7 mg/l. Hypocotyl and leaf-derived protoplasts formed microcolonies. Addition of CaCl2 and glutamine to the basic medium improved protoplast survival, and division of hypocotyl and leaf-derived protoplasts. The growth conditions, such as the light and carbon source of donor plant were the important factors for protoplast isolation and culture. |